Get the answer to your homework problem. Try Numerade free for 7 days
Julie S. Biology 1 year, 1 month ago
Understanding: • The replication of DNA is semi-conservative and depends on complementary base pairing
Semi-Conservative DNA Molecule
This occurs because each nitrogenous base can only pair with its complementary partner
Conservation of Sequence by Complementary Base Pairing
Skill: • Analysis of Meselson and Stahl’s results to obtain support for the theory of semi-conservative replication of DNA
Three Proposed Models of DNA Replication
Meselson and Stahl were able to experimentally test the validity of these three models using radioactive isotopes of nitrogen
Results of the Meselson-Stahl Experiment
Semiconservative replication describe the mechanism of DNA replication in all known cells. DNA replication occurs on multiple origins of replication along the DNA template strand (antinsense strand). As the DNA double helix is unwound by helicase, replication occurs separately on each template strand (sense strand) in antiparallel directions. This process is known as semi-conservative replication because two copies of the original DNA molecule are produced, each copy conserving (replicating) the information from one half of the original DNA molecule.[1] [2] Each copy contains one original strand and one newly-synthesized strand. (Both copies should be identical, but this is not entirely assured.) The structure of DNA (as deciphered by James D. Watson and Francis Crick in 1953) suggested that each strand of the double helix would serve as a template for synthesis of a new strand. It was not known how newly synthesized strands combined with template strands to form two double helical DNA molecules.[3][4] Meselson-Stahl experiment using isotopes to discover semiconservative replication. Multiple experiments were conducted to determine how DNA replicates. The semiconservative model was anticipated by Nikolai Koltsov and later supported by the Meselson-Stahl experiment.,[4][5] which confirmed that DNA replicated semi-conservatively by conducting an experiment using two isotopes: nitrogen-15 (15 Three postulated methods of DNA synthesis Semiconservative replication derives its name from the fact that this mechanism of transcription was one of three models originally proposed[3][4] for DNA replication:
For semiconservative replication to occur, the DNA double-helix needs to be separated so the new template strand can be bound to the complementary base pairs. Topoisomerase is the enzyme that aids in the unzipping and recombination of the double-helix. Specifically, topoisomerase prevents the double-helix from supercoiling, or becoming too tightly wound. Three topoisomerase enzymes are involved in this process: Type IA Topoisomerase, Type IB Topoisomerase, and Type II Topoisomerase.[9] Type I Topoisomerase unwinds double stranded DNA while Type II Topoisomerase breaks the hydrogen bonds linking the complementary base pairs of DNA.[8] The rate of semiconservative DNA replication in a living cell was first measured as the rate of the T4 phage DNA strand elongation in phage-infected E. coli.[10] During the period of exponential DNA increase at 37 °C, the rate of strand elongation was 749 nucleotides per second. The mutation rate per base pair per round of replication during phage T4 DNA synthesis is 2.4×10−8.[11] Thus, semiconservative DNA replication is both rapid and accurate. Semiconservative replication provides many advantages for DNA. It is fast, accurate, and allows for easy repair of DNA. It is also responsible for phenotypic diversity in a few prokaryotic species.[12] The process of creating a newly synthesized strand from the template strand allows for the old strand to be methylated at a separate time from the new strand. This allows repair enzymes to proofread the new strand and correct any mutations or errors.[7] DNA could have the ability to activate or deactivate certain areas on the newly synthesized strand that allows the phenotype of the cell to be changed. This could be advantageous for the cell because DNA could activate a more favorable phenotype to aid in survival. Due to natural selection, the more favorable phenotype would persist throughout the species. This gives rise to the idea of inheritance, or why certain phenotypes are inherited over another.[7]
|