What is the process that destroys the non spore forming contaminants on inanimate objects is?

Sterilization describes a process that destroys or eliminates all forms of microbial life and is carried out in health-care facilities by physical or chemical methods. Steam under pressure, dry heat, EtO gas, hydrogen peroxide gas plasma, and liquid chemicals are the principal sterilizing agents used in health-care facilities. Sterilization is intended to convey an absolute meaning; unfortunately, however, some health professionals and the technical and commercial literature refer to “disinfection” as “sterilization” and items as “partially sterile.” When chemicals are used to destroy all forms of microbiologic life, they can be called chemical sterilants. These same germicides used for shorter exposure periods also can be part of the disinfection process (i.e., high-level disinfection).

Disinfection describes a process that eliminates many or all pathogenic microorganisms, except bacterial spores, on inanimate objects (Tables 1 and 2). In health-care settings, objects usually are disinfected by liquid chemicals or wet pasteurization. Each of the various factors that affect the efficacy of disinfection can nullify or limit the efficacy of the process.

Factors that affect the efficacy of both disinfection and sterilization include prior cleaning of the object; organic and inorganic load present; type and level of microbial contamination; concentration of and exposure time to the germicide; physical nature of the object (e.g., crevices, hinges, and lumens); presence of biofilms; temperature and pH of the disinfection process; and in some cases, relative humidity of the sterilization process (e.g., ethylene oxide).

Unlike sterilization, disinfection is not sporicidal. A few disinfectants will kill spores with prolonged exposure times (3–12 hours); these are called chemical sterilants. At similar concentrations but with shorter exposure periods (e.g., 20 minutes for 2% glutaraldehyde), these same disinfectants will kill all microorganisms except large numbers of bacterial spores; they are called high-level disinfectants. Low-level disinfectants can kill most vegetative bacteria, some fungi, and some viruses in a practical period of time (≤10 minutes). Intermediate-level disinfectants might be cidal for mycobacteria, vegetative bacteria, most viruses, and most fungi but do not necessarily kill bacterial spores. Germicides differ markedly, primarily in their antimicrobial spectrum and rapidity of action.

Cleaning is the removal of visible soil (e.g., organic and inorganic material) from objects and surfaces and normally is accomplished manually or mechanically using water with detergents or enzymatic products. Thorough cleaning is essential before high-level disinfection and sterilization because inorganic and organic materials that remain on the surfaces of instruments interfere with the effectiveness of these processes. Decontamination removes pathogenic microorganisms from objects so they are safe to handle, use, or discard.

Terms with the suffix cide or cidal for killing action also are commonly used. For example, a germicide is an agent that can kill microorganisms, particularly pathogenic organisms (“germs”). The term germicide includes both antiseptics and disinfectants. Antiseptics are germicides applied to living tissue and skin; disinfectants are antimicrobials applied only to inanimate objects. In general, antiseptics are used only on the skin and not for surface disinfection, and disinfectants are not used for skin antisepsis because they can injure skin and other tissues. Virucide, fungicide, bactericide, sporicide, and tuberculocide can kill the type of microorganism identified by the prefix. For example, a bactericide is an agent that kills bacteria. 13-18

A microbicidal agent does what? a. kills spores b. inhibits microorganisms c. is ineffective against viruses

d. destroys microbes

Microbial control methods that kill ____ are able to sterilize. a. viruses b. the tubercle bacillus c. endospores

d. cysts

Any process that destroys the non-spore-forming contaminants on inanimate objects is: a. antisepsis b. disinfection c. sterilization

d. degermation

Sanitation is a process by which: a. the microbial load on objects is reduced b. objects are made sterile with chemicals c. utensils are scrubbed

d. skin is debrided

a. the microbial load on objects is reduced

An example of an agent that lowers the surface tension of cells is: a. phenol b. chlorine c. alcohol

d. formalin

High temperatures ___ and low temperatures ____ a. sterilize, disinfect b. kill cells, inhibit cell growth c. denature proteins, burst cells

d. speed up metabolism, slow down metabolism

b. kill cells, inhibit cell growth

The temperature-pressure combination for an autoclave is: a. 100 degrees C and 4 psi b. 121 degrees C and 15 psi c. 131 degrees C and 9 psi

d. 115 degrees C and 3 psi

b. 121 degrees C and 15 psi

Microbes that are the targets of pasteurization: a. Clostridium botulinum b. Mycobacterium species c. Salmonella species

d. both b and c

Ionizing radiation removes ___ from atoms. a. protons b. waves c. electrons

d. ions

The primary ode of action of nonionizing radiation is to: a. produce superoxide ions b. make pyrimidine dimers c. denature proteins

d. break disulfide bonds

b. make pyrimidine dimers

An effective method of sterilizing heat-sensitive liquids is: a. UV radiation b. exposure to ozone c. beta propiolacteone

d. filtration

____ is the iodine antiseptic of choice for wound treatment a. Eight percent tincture b. Five percent aqueous c. Iodophor

d. Potassium iodine solution

A chemical with sporicidal properties is: a. phenol b. alcohol c. quaternary ammonium compound

d. glutaraldehyde

Silver nitrate is used: a. in antisepsis of burns b. as a mouthwash c. to treat genital gonorrhea

d. to disinfect water

a. in antisepsis of burns

Detergents are: a. high-level germicides b. low-level germicides c. excellent antiseptics

d. used in disinfecting surgical instruments