Thyroid hormone is the hormone that’s mainly responsible for controlling the speed of your body’s metabolism. In infants, thyroid hormone is critical for brain development. Your thyroid, a small, butterfly-shaped gland located at the front of your neck under your skin, makes and releases thyroid hormone. It’s a part of your endocrine system.
Hormones are chemicals that coordinate different functions in your body by carrying messages through your blood to your organs, muscles and other tissues. These signals tell your body what to do and when to do it.
Metabolism is the complex process of how your body transforms the food you consume into energy. All of the cells in your body need energy to function.
Thyroid hormone actually represents the combination of the two main hormones that your thyroid gland releases: thyroxine (T4) and triiodothyronine (T3). They’re often collectively referred to as “thyroid hormone” because T4 is largely inactive, meaning it doesn’t impact your cells, whereas T3 is active. Once your thyroid releases T4, certain organs in your body transform it into T3 so that it can impact your cells and your metabolism.
Your thyroid also releases a hormone called calcitonin to help regulate calcium levels in your blood by decreasing it. Calcitonin isn’t grouped into the “thyroid hormone” name, and it doesn’t impact your body’s metabolism like T3 and T4 do.
How are thyroid hormone levels controlled?
The production and release of thyroid hormone — thyroxine (t4) and triiodothyronine (T3) — is controlled by a feedback loop system that involves the following:
- Hypothalamus.
- Pituitary gland.
- Thyroid gland.
- Multiple hormones.
Your hypothalamus is the part of your brain that controls functions like blood pressure, heart rate, body temperature and digestion.
Your pituitary gland is a small, pea-sized gland located at the base of your brain below your hypothalamus. It makes and releases eight hormones.
Your pituitary gland is connected to your hypothalamus through a stalk of blood vessels and nerves. This is called the pituitary stalk. Through the stalk, your hypothalamus communicates with your pituitary gland and tells it to release certain hormones.
To start the feedback loop, your hypothalamus releases thyroid-releasing hormone (TRH) which, in turn, stimulates your pituitary gland to produce and release thyroid-stimulating hormone (TSH). TSH then triggers your thyroid to produce T4 and T3. Of the total amount of hormones that TSH triggers your thyroid to release, about 80% is T4 and 20% is T3. Your thyroid also needs adequate amounts of iodine, a substance you get from the food you eat, to create T4 and T3.
This hormone chain reaction is regulated by a feedback loop so that when the levels of T3 and T4 increase, they prevent the release of TRH (and thus TSH). When T3 and T4 levels drop, the feedback loop starts again. This system allows your body to maintain a constant level of thyroid hormones in your body.
If there are any issues with your hypothalamus, pituitary gland or thyroid, it can result in an imbalance in the hormones involved in this system, including T3 and T4.
What is the function of thyroid hormone?
Once your thyroid releases thyroxine (T4) into your bloodstream, certain cells in your body transform it into triiodothyronine (T3) through a process called de-iodination. This is because cells that have receptors that receive the effect of thyroid hormone are better able to use T3 than T4. Therefore, T4 is generally considered to be the inactive form of thyroid hormone, and T3 is considered the active form of it.
Cells in the following tissues, glands, organs and body systems can convert T4 to T3:
- Liver.
- Kidneys.
- Muscles.
- Thyroid.
- Pituitary gland.
- Brown adipose (fat) tissue (This type of fat produces heat to help maintain your body temperature in cold conditions).
- Central nervous system.
Thyroid hormone (T3 and T4) affects every cell and all the organs in your body by:
- Regulating the rate at which your body uses calories (energy). This affects weight loss or weight gain and is called the metabolic rate.
- Slowing down or speeding up your heart rate.
- Raising or lowering your body temperature.
- Influencing the speed at which food moves through your digestive tract.
- Affecting brain development.
- Controlling the way your muscles contract.
- Managing skin and bone maintenance by controlling the rate at which your body replaces dying cells (a normal process).
What tests measure thyroid levels?
Several blood tests can measure your thyroid levels and assess how well your thyroid is working. These tests are often called thyroid function tests and include:
- Total T4 (thyroxine) test.
- Free T4 (FT4) test.
- Total T3 (triiodothyronine) test.
- Free T3 (Ft3) test.
- Thyroid-stimulating hormone (TSH) test.
Your provider may order additional tests to assess your thyroid function, including:
- Thyroid antibodies: These tests help identify different types of autoimmune thyroid conditions.
- Thyroglobulin: This test is used to diagnose thyroiditis (thyroid inflammation) and to monitor the treatment of thyroid cancer.
Several conditions can result from or cause abnormal thyroid hormone levels. Thyroid disease is very common, with an estimated 20 million people in the United States having some type of thyroid condition. A person assigned female at birth is about five to eight times more likely to have a thyroid condition than a person assigned male at birth.
Thyroid conditions include:
Issues with your pituitary gland or hypothalamus can also cause abnormal thyroid hormone levels since they help control thyroid hormone levels.
When should I see my doctor about my thyroid hormone levels?
Abnormal thyroid hormone levels usually cause noticeable symptoms. Since thyroid hormone is responsible for controlling the speed of your metabolism, too much thyroid hormone can make it faster than normal and too little thyroid hormone can slow it down. These imbalances cause certain symptoms, including:
- Unexplained weight gain or weight loss.
- Slow or fast heart rate.
- Intolerance to cold or heat.
- Dry or moist skin.
- Irregular menstrual cycles.
If you experience these symptoms, contact your healthcare provider. They can run some simple blood tests to see if your thyroid hormone levels are irregular.
A note from Cleveland Clinic
Thyroid hormone is an essential hormone that affects many aspects of your body. Sometimes, you can have too little or too much thyroid hormone. The good news is that thyroid conditions are highly treatable. If you’re experiencing any thyroid hormone-related symptoms or want to know if you have any risk factors for developing thyroid disease, don’t be afraid to talk to your healthcare provider. They’re there to help you.
Thyroid-stimulating hormone, also known as TSH, is a glycoprotein hormone produced by the anterior pituitary. It is the primary stimulus for thyroid hormone production by the thyroid gland. It also exerts growth effects on thyroid follicular cells leading to enlargement of the thyroid. The hypothalamic-pituitary axis regulates TSH release. Specifically, neurons in the hypothalamus release TRH, or thyroid-releasing hormone, which stimulates thyrotrophs of the anterior pituitary to secrete TSH. TSH, in turn, stimulates thyroid follicular cells to release thyroid hormones in the form of T3 or T4. Triiodothyronine, or T3, is the active form of thyroid hormone. Though it represents only 20% of the released hormone, the majority of T3 comes from the peripheral conversion of T4 to T3. Tetraiodothyronine, also known as thyroxine or T4, constitutes more than 80% of the secreted hormone. When released into the circulation, it forms T3 through the process of de-iodination. T4 and T3 can then exert negative feedback on the anterior pituitary with high levels of T3/T4 decreasing TSH secretion and low levels of T3/T4 increasing TSH release. In this review, we discuss the physiology, biochemistry, and clinical relevance of TSH.[1]
Primary thyroid disease refers to problems arising from the thyroid gland itself. In contrast, secondary thyroid disease refers to central problems arising from the anterior pituitary that indirectly affects thyroid function. A thyroid problem can exist in the form of hyperthyroidism or hypothyroidism. Hyperthyroidism occurs when there is excessive thyroid hormone synthesis or release. Hypothyroidism, on the other hand, happens due to inadequate thyroid hormone production.
In primary hyperthyroidism, the thyroid produces large amounts of T3 and T4, which, through negative feedback inhibition, suppress TSH secretion from the anterior pituitary. In primary hypothyroidism, the thyroid produces insufficient amounts of T3 and T4, which leads to loss of negative feedback inhibition, and increased production of TSH from the anterior pituitary. In secondary hyperthyroidism, the anterior pituitary produces large amounts of TSH, which, in turn, stimulate the thyroid follicular cells to secrete thyroid hormones in excessive amounts. On the other hand, if the anterior pituitary were to produce low levels of TSH, lack of stimulation of thyroid follicular cells causes T3 and T4 levels to go down, thus secondary hypothyroidism.
TSH is the first-line screening test for the majority of patients with a suspected thyroid problem. Together, with T3 and T4, it helps assess whether thyroid disease is primary or secondary. Thyroid function tests measure the levels of T3, T4, and TSH in the blood. They are critical not only for diagnosing thyroid problems but also in differentiating between a primary and a secondary cause of thyroid disease. A change in TSH that parallels T3 and T4 changes indicates a secondary problem originating in the anterior pituitary. In contrast, a TSH change that follows the opposite direction of T3 and T4 suggests a problem in the thyroid gland itself.[2][3][4]
TSH is a peptide hormone produced by the anterior pituitary. It consists of two chains: an alpha chain and a beta chain. It has a molecular mass of approximately 28,000 Da. The composition is very similar to other glycoprotein hormones made by the anterior pituitary. Luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (HCG) very much resemble TSH. Specifically, they all have the same alpha subunit as TSH, but different beta chains that confer biological specificity. Since TSH, LH, FSH, and HCG share the same alpha subunit, they all function through the same cyclic adenine monophosphate (cAMP) second messenger system. The cAMP second messenger system entails adenine monophosphate (AMP) conversion to cAMP. In addition to cAMP, TSH also activates the IP3 signaling cascade. The IP3 second messenger system involves calcium release from the sarcoplasmic reticulum. Both cAMP and IP3/Ca2+ cascades lead to downstream physiological effects that enhance thyroid hormone synthesis and thyroid gland growth.
The primary target of TSH is the thyroid gland. Specifically, TSH modulates the release of T3 and T4 from thyroid follicular cells. Around 80% of the thyroid hormone is released as T4. T4 is de-iodinated to T3, which is a more potent thyroid hormone. Even though only about 20% of T3 originates from the thyroid gland, 80% comes from peripheral conversion via a deiodinase. More than 99% of thyroid hormones bind to thyroid-binding globulin, prealbumin, and albumin, and only 1% circulates freely in the blood. Once T3 binds to its receptor in the nucleus, it activates DNA transcription, followed by mRNA translation, and new protein synthesis. These new proteins influence many organ systems, promoting growth as well as bone and central nervous system (CNS) maturation. T3 and T4 act on almost all cells in the body to increase the basal metabolic rate. Specifically, they increase the synthesis of Na?/K?-ATPase, leading to an increase in oxygen consumption and heat production. They also act on B1 receptors in the heart to increase heart rate and contractility through increasing the number of beta-1 receptors on the myocardium such that the myocardium is more sensitive to stimulation by the sympathetic nervous system. Thyroid hormones also activate metabolism, with an increase in glucose absorption, glycogenolysis, gluconeogenesis, lipolysis, and protein synthesis and degradation (net catabolic).[5]
TSH binds to and activates the TSH receptor (TSHR), which is a G-protein coupled receptor (GPCR) on the basolateral surface of thyroid follicular cells. TSHR is coupled to both Gs and Gq G-proteins, activating both the cAMP pathway (via Gsa) and the phosphoinositol/calcium (IP/Ca2+; via Gq) second messenger signaling cascades. The Gs pathway activates iodide uptake, thyroid hormone secretion, and gland growth and differentiation. The Gq pathway is rate-limiting for hormone synthesis by stimulating iodide organification. A gain in function mutation of the TSH receptor results in hyperthyroidism, while a loss in function mutation results in hypothyroidism.
Understanding the role of TSH in stimulating T3 and T4 secretion requires knowledge of the thyroid hormone synthesis pathway. The two main components of T3 and T4 are iodine and tyrosine. Iodine (I2) forms through oxidation of iodide (I-) after thyroid follicular cells actively take up iodide (I-) from the bloodstream against its concentration gradient. Tyrosine, on the other hand, comes from thyroglobulin, a tyrosine-rich protein synthesized by thyroid follicular cells. Following iodide uptake and oxidation, iodine binds tyrosine residues on thyroglobulin to form monoiodotyrosine (MIT) and diiodotyrosine (DIT). Triiodothyronine, or T3, forms when an MIT mixes up with DIT. Likewise, the coupling of two DITs creates tetraiodothyronine (T4), or thyroxine. The process of iodide oxidation, thyroglobulin iodination, and MIT and DIT coupling is catalyzed by an enzyme called, thyroperoxidase. TSH stimulates thyroid hormone secretion through enhancing iodide uptake, thyroglobulin synthesis, and thyroperoxidase activity. Additionally, TSH also increases blood flow to the thyroid gland and stimulates hypertrophy and hyperplasia of thyroid follicular cells to exert growth effects on the thyroid gland.[6]
The hypothalamic-pituitary axis regulates TSH release through hypothalamic neurons that secrete thyroid releasing hormone (TRH), a hormone that stimulates thyrotrophs in the anterior pituitary to secrete TSH. TSH, in turn, stimulates thyroid follicular cells to release thyroxine or T4 (80%), and triiodothyronine or T3 (20%). Somatostatin, on the other hand, is another hormone produced by the hypothalamus that inhibits the release of TSH from the anterior pituitary. When T4 enters the circulation, it gets converted to T3 through the process of deiodination. T4 and T3 can then exert negative feedback on TSH levels (high levels of T3/T4 decrease TSH release from the anterior pituitary, while low levels of T3/T4 increase TSH release). T3 is the predominant inhibitor of TSH secretion. Because TSH secretion is so sensitive to minor changes in free T4 through this negative feedback loop, abnormal TSH levels are detected earlier than those of free T4 in hypothyroidism and hyperthyroidism. There is a log-linear relationship between T3/T4 and TSH, and minor changes in T3/T4 lead to significant changes in TSH.
TSH is the first-line screening test for both hypothyroidism and hyperthyroidism since changes in TSH occur earlier that changes in T3/T4. If values are outside the range of 0.4 to 4.5 milliunits per liter (mU/L), measuring T3 and T4 should follow. However, TSH is always the best first test because it is more reliable than plasma T3/T4 levels, which tend to fluctuate. In primary hypothyroidism, TSH levels are elevated due to the loss of negative inhibition on the anterior pituitary. In contrast, in primary hyperthyroidism, TSH levels go down.
Another relevant test when it comes to thyroid disease is thyroid-stimulating immunoglobulin (TSI). The test is diagnostic for a condition called Grave's disease. As discussed later, Grave's disease is an autoimmune disease characterized by the presence of autoantibodies in the blood that exert TSH-like effects. The antibodies stimulate TSHR on thyroid follicular cells leading to uncontrolled thyroid hormone synthesis and release. The test detects the presence of thyroid-stimulating antibodies (TSIs) in the blood.[7]
Hyperthyroidism
Hyperthyroidism is a condition characterized by excessive secretion of thyroid hormones. A variety of medical conditions lead to hyperthyroidism, including Graves' disease, thyroid neoplasm, thyroid adenomas, excess TSH secretion, or exogenous T3 or T4 administration. Symptoms of hyperthyroidism include increased basal metabolic rate, weight loss, increased appetite, sweating, tremors, heat sensitivity, irritability, diarrhea, and insomnia. In primary hyperthyroidism, as in the case of a thyroid adenoma, TSH levels tend to decrease due to negative feedback inhibition exerted on the anterior pituitary by T3 and T4. In secondary hyperthyroidism, as in the case of a TSH or TRH secreting tumor, both TSH and T3/T4 levels increase. [8][9]
Hypothyroidism
Hypothyroidism occurs when the thyroid gland fails to produce thyroid hormone in sufficient amounts. The most common cause of hypothyroidism is Hashimoto thyroiditis, which is a condition caused by autoantibodies that attack thyroid follicular cells leading to decreased thyroid hormone synthesis. Other common causes of hypothyroidism include radiation therapy, thyroid surgery, overtreatment with anti-thyroid medications, congenital hypothyroidism, iodine deficiency, or pituitary tumors. Symptoms of hypothyroidism include decreased basal metabolic rate, weight gain despite the decreased appetite, cold sensitivity, decreased cardiac output, hypoventilation, lethargy and mental slowness, drooping eyelids, myxedema, growth retardation, mental retardation in perinatal patients, and goiter. In primary hypothyroidism, as in the case of Hashimoto thyroiditis, TSH levels increase due to loss of negative feedback inhibition. In secondary hypothyroidism, as in the case of a benign pituitary gland tumor, TSH levels go down. Treatment for hypothyroidism includes thyroid hormone replacement therapy.[10][11][12]
TSH and Estrogen
Thyroxine-binding globulin (TBG) binds most T3/T4 in the blood. A small portion of thyroid hormones circulate freely in the blood and constitute the physiologically active form. Estrogen increases the synthesis and decreases the clearance of thyroxine-binding globulin (TBG). Therefore, excess estrogen states, such as pregnancy or the use of oral contraceptives, cause TBG levels to increase. An increase in binding activity (increased TBG) initially lowers the concentration of the free hormone. However, an intact hypothalamic-pituitary-thyroid axis quickly normalizes free hormone levels and restores homeostasis. As such, TSH and free T3/T4 levels remain normal while Total T3/T4 increases. In contrast, patients with pre-existing hypothyroidism rely on exogenous thyroid hormone (levothyroxine) to maintain adequate free T3/T4 levels. As such, they fail to respond appropriately to a drop in free thyroid hormone levels. Therefore, an increase in TBG in those patients would cause a decrease in free T3/T4. The loss of negative feedback inhibition on the pituitary causes TSH levels to go up. Those patients require an increase in levothyroxine dosage to maintain euthyroidism.
Grave's Disease
Grave's disease is an autoimmune condition that most commonly affects young women under the age of forty. The hallmark about the disease is the presence of autoantibodies that exert TSH-like effects, called thyroid-stimulating antibodies (TSIs). TSIs stimulate TSH receptors on thyroid follicular cells leading to both hyperthyroidism and thyroid gland enlargement, hence a goiter. As T3/T4 levels increase, TSH goes down through negative feedback inhibition. In addition to the classical signs and symptoms of hyperthyroidism, Grave's disease also causes ophthalmopathy, which manifests as exophthalmos (bulging eyes), eye irritation, double vision, and possibly vision loss. Grave's ophthalmopathy most likely happens due to TSIs binding to receptors on the soft tissues and muscles behind the eyes. This initiates an inflammatory cascade that causes ocular symptoms to occur.[8]
TSH-secreting Pituitary Adenoma
A functioning pituitary adenoma is a pituitary tumor that secretes active hormones. It usually arises from prolactin-secreting cells, also known as lactotrophs. It can also arise from TSH-secreting thyrotrophs leading to a rise in TSH levels. TSH-secreting adenomas are often macroadenomas that not only produce excessive amounts of TSH but also create mass-effects on adjacent structures. Patients usually complain of compressive symptoms (headaches, vision problems) as well as symptoms of hyperthyroidism. High TSH causes the thyroid gland to enlarge, and patients typically develop a goiter.[13]
Non-functioning Pituitary Adenoma
A non-functioning pituitary adenoma usually arises from gonadotrophs (gonadotropin-secreting cells). Healthy gonadotrophs secrete active LH and FSH, consisting of both alpha and beta-subunits. In contrast, a non-functioning pituitary adenoma secretes an inactive form of gonadotropins that has the alpha chain but lacks the active beta-subunit. Symptoms usually do not occur until the tumor becomes large enough to compress adjacent structures. Common compressive symptoms include headaches and vision problems. Additionally, the large mass might compress the adjacent pituitary cells leading to decreased TSH as well as other pituitary hormones. A drop in TSH levels might lead to hypothyroidism if the tumor is not adequately managed.[14]
Review Questions
1.
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